Cryopreservation and Freeze-Drying Protocols
نویسندگان
چکیده
This book explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens from humans, animals, plants that are research purposes, conservation of genetic reserves, applications in agriculture medicine.
منابع مشابه
Circulating-Gas Freeze Drying
WAGMAN, JACK (U.S. Army Biological Laboratories, Frederick, Md.) AND EDWARD J. WENECK. Preservation of bacteria by circulating-gas freeze drying. Appl. Microbiol. 11:244-248. 1963.-Water-washed Serratia marcescens and Escherichia coli were freeze dried in a circulating-gas system at atmospheric pressure. This convective procedure resulted in a substantially higher survival of organisms than cou...
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Resumen Cuatro hongos basidiomycetes, Agaricus blazei Murrill (Agaricomycetideae), Ganoderma lucidum (W.Curt.: Fr.) P. Karst. y Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes), y Pleurotus pulmonarius (Fr.) Quél. (Agaricomycetideae) fueron evaluados bajo tres métodos de conservación durante 12 meses, observando su viabilidad con el fin de establecer el mejor método de conservac...
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Today, sperm cryopreservation is a routine practice in infertility clinics. One of the alternative methods to preserve sperm is freeze-drying. This process consists of three steps. The first step is sample freezing, during which the solvent is separated from the solvent. Water forms ice crystals and the solute is placed between the ice crystals. Freezing may be done in a separate device or in a...
متن کاملFreeze-drying above room temperature.
This study investigates the use of solid, organic compounds to lyophilize drugs without conventional freeze-drying equipment. The aim of the investigation is to find a pharmaceutically acceptable solvent or solvent combination that is appropriate for freeze-drying on the basis of its ability to (1) solubilize hydrophobic drugs, (2) provide a stable environment for water-sensitive compounds, (3)...
متن کاملFreeze-drying of Aegyptianella pullorum.
Heparinized whole blood, parasitized with Aegyptianella pullorum, was collected from 2 fowls. Buffered lactose peptone (BLP) was added v/v as a stabilizer and the mixture lyophilized in 2 ml aliquots after rapid or slow freezing. At different stages during the freeze-drying process, as well as after lyophilization and reconstitution with 1.8 ml of sterile water, samples were taken and injected ...
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ژورنال
عنوان ژورنال: Methods in molecular biology
سال: 2021
ISSN: ['1940-6029', '1064-3745']
DOI: https://doi.org/10.1007/978-1-0716-0783-1